Chin J Cancer Biother, Sep. 2023, Vol. 30, No.9

[Abstract]  Objective: To investigate the effects of lycopene on the proliferation and apoptosis of renal carcinoma 786-O cells through  silent information regulator 1 (SIRT1)/nuclear factor-kappa B (NF-κB) axis. Methods: Normal human kidney HK-2 cells and human  renal cancer 786-O cells were conventionally cultured. The experiment included control group (0.1% DMSO), cisplatin group (40 μg/mL),  lycopene low concentration (2.5 μg/mL) group, lycopene high concentration (5 μg/mL) group, and lycopene high concentration  (5 μg/mL)+ EX527 (SIRT1 inhibitor) (3 µmol/L) group. The proliferation capacity of 786-O cells in each treatment group was detected  by CCK-8 method and clonal formation assay. The apoptosis of HK-2 and 786-O cells in each treatment group was detected by flow  cytometry. The changes of mitochondrial membrane potential and reactive oxygen species (ROS) in 786-O cells in each treatment group  were detected by RH123 and DCFH-DA staining, respectively. The expression of apoptosis-related proteins, BAX, Bcl-2, C-casp3 and  SIRT1/NF-κB axis-related proteins SIRT1 and p-NF-Κb, in 786-O cells were detected by WB method. The effects of low concentration  lycopene (5 mg/kg), high concentration lycopene (20 mg/Kg), cisplatin (2 mg/kg), and lycopene (20 mg/kg) +EX527 (10 mg/kg) on the  growth of 786-O cell transplanted tumor were observed. TUNEL method was used to detect the apoptosis in the tissues of transplanted  tumors in each group. Results: Lycopene inhibited the proliferation activity of 786-O cells in a dose dependent manner. Lycopene and  cisplatin significantly inhibited the clonogenesis ability of 786-O cells and promoted their apoptosis; moreover, after lycopene and  cisplatin treatment, the MMP level was increased while ROS level was decreased, the expression of apoptosis-related proteins BAX and  C-casp3 were significantly increased (all P<0.05), while the expression of Bcl-2 was down-regulated (P<0.05), the expression of SIRT1  was significantly increased (all P<0.05) and the expression of p-NF-κB was significantly decreased (all P<0.05). These effects could be  reversed by EX527. Lycopene and cisplatin inhibited the growth of 786-O cell grafts in vivo and promoted the apoptosis of tumor cells,and their effects could also be reversed by EX527. Conclusion: Lycopene may inhibit the activation of NF-κB pathway through up regulation of SIRT1, thus inhibiting proliferation of 786-O cells and inducing apoptosis. 

本研究中对照品：番茄红素 购自于四川恒诚致远生物科技有限公司（Naturewill biotechnology Co., Ltd.）